IVF

Fertilization in vitro is an auxiliary reproductive therapeutic technique in which the egg is fertilized by the sperm in the laboratory. Usually the technique consists of 3 main stages: - induction of ovulation (stimulation of the ovaries); - Egg production, fertilization and early embryo cultivation; - transfer of embryos into the uterine cavity. IVF or in vitro fertilization is considered a standard method of assisted fertilization. The essence of it is that we create conditions for the contact of one egg and more than 100 thousand spermatozoa and we expect the onset of fertilization. Before the advent of ICSI, this was the only way to IVF (in vitro fertilization). At present, in the classical form, this method is almost not used.

ICSI

This is the process of introducing spermatozoa into the cytoplasm of the egg with the help of microinjects. Selection of spermatozoa is carried out under a microscope with a 400-fold increase. To date, the most commonly used method of assisted fertilization./

IMSI

Under such an increase, unlike ICSI, an increase (optical or electronic) up to 8,000 times is used here. Under such an increase, defects in spermatozoa that can not be seen in an ordinary microscope become visible, despite the obvious theoretical and clinical justifications of this method, there is no unambiguous evidence of a higher efficiency of IMSI cycles compared to ICSI cycles. Thus, the use of IMSI is more often recommended in cases of severe teratospermia or with proven fragmentation of sperm DNA.

 

When do recommend assisted insemination?

In the usual cycle, 10-15 follicles grow, but only 1 of them ripens, and the ovum ovulates. The rest of the follicles regress, and the eggs in them die. In the program of assisted fertilization, our goal is to allow several ovules to ripen right away. This increases your chances of successful treatment. For the induction of ovulation in the IVF cycle, we use preparations of analogues of your own controlled hormones - follicle stimulating (FSH) and luteinizing (LH), these substances are involved in the natural process of ovulation. The drugs are administered subcutaneously and less often intramuscularly, you can inject yourself at home or in our clinic. Induction of ovulation lasts from 8 to 12 days. Under the supervision of ultrasound, we observe the growth of follicles and, if necessary, control the levels of blood hormones. In total during the stimulation period you will visit the clinic 3 - 4 times.

How do stimulate the ovaries?

In the usual cycle, 10-15 follicles grow, but only 1 of them ripens, and the ovum ovulates. The rest of the follicles regress, and the eggs in them die. In the program of assisted fertilization, our goal is to allow several ovules to ripen right away. This increases your chances of successful treatment. For the induction of ovulation in the IVF cycle, we use preparations of analogues of your own controlled hormones - follicle stimulating (FSH) and luteinizing (LH), these substances are involved in the natural process of ovulation. The drugs are administered subcutaneously and less often intramuscularly, you can inject yourself at home or in our clinic. Induction of ovulation lasts from 8 to 12 days. Under the supervision of ultrasound, we observe the growth of follicles and, if necessary, control the levels of blood hormones. In total during the stimulation period you will visit the clinic 3 - 4 times.

When do you need sperm?

With assistive fertilization, in most cases, partner sperm is used. Sperm donor is used less seldom if all the requirements of the law are met and selected in accordance with the patient's request. Seminal fluid is obtained by masturbation on the day of oocyte oocyte collection. Cryopreserved semen can also be used if necessary. In the case of using donor sperm, its samples are always frozen. A rare, but also used method of collecting semen is aspiration (TESA) or biopsy (TESE) of testicular tissue. Both these technologies are used in the case of complete suction of spermatozoa in the ejaculate.

How do they collect eggs?

Eggs are obtained during ovarian puncture, which is performed under the supervision of ultrasound. This is a simple intervention that is conducted under anesthesia. She does not experience any painful sensations. In this case, there is no any aftereffect and effects that characterize the longer use of anesthesia. Since this is an outpatient procedure, the patient will be able to go home after 1, 5 - 2 hours after collecting the eggs.

What happens to the egg between fertilization and transfer to the uterus?

After fertilization of the oocyte, we observe their development in the conditions of the embryological laboratory. All this time, embryos and eggs are stored in special incubators simulating the environment in the uterine cavity and creating ideal conditions for the development of embryos. After 18-20 hours we control the onset of fertilization. Daily development of each embryo is observed up to 5 days, when embryos are transferred to the uterine cavity. This long cultivation up to 5 days allows us to select embryos (blastocysts) with the greatest capacity for implantation, thus increasing the chances of pregnancy.